Composite
OsmC

Part:BBa_K2832006

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


RcsB/NhaR mRFP1 Generator ( RcsB/NhaR ---> OsmCp ---> mRFP1 ) RcsB and NhaR are transcription factors that bind to osmC promoter sequence. rcsB is responsible for cell division, periplasmic proteins, motility, and biofilm formation. rcsB described as “global regulator controlling cell surface composition in response to changing environment.” Sound could play role in antagonizing one of these systems to activate rcsB. NhaR is dependent on Na+ and controls genes for defense against Na+, alkaline pH environments, adverse conditions, and biofilm formation. Again this represented a large array of systems that sound may antagonize. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates the expression of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 829
    Illegal AgeI site found at 941
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None